A Ruthenium(II) Polypyridyl Nucleoside as a Potential Photodynamic Therapy Agent

作者：Chen, Jun; Li, Kaiyu; Swavey, Shawn^*; Church, Kevin M.

来源：ChemistrySelect, 2016, 1(4): 793-797.

DOI：10.1002/slct.201600126

• Metal-organic frameworks
• transition metals
• nucleoside
• photodynamic therapy
• ruthenium

A new ruthenium(II) nucleoside Ru(II)-PPY has been synthesized and characterized by NMR and high resolution mass spectrometry. This complex couples a ruthenium(II) polypyridyl light absorber to a nucleoside through a pyridyl group. The absorption spectroscopy of the complex shows a Ru(II) to bipyridyl metal-to-ligand charge transfer (MLCT) transition in the visible region of the spectrum. Cyclic voltammetry of the nucleoside and the ruthenium(II) nucleoside confirms coordination of the ruthenium moiety to the nucleoside with a quasi-reversible Ru(III/II) redox couple of E-1/ 2 = 0.82 V vs. Ag/AgCl. Irradiation of buffered solutions of plasmid DNA and the Ru(II)-PPY complex using a long band pass filter to block out ultraviolet light indicate that the complex is capable of photo-nicking the dsDNA. Additional irradiation experiments in the presence of singlet oxygen and superoxide inhibitors suggest that the Ru(II)-PPY complex generates both singlet oxygen and oxygen radicals when photo-excited.

• 出版日期2016-4