A Novel Alkaline Hemicellulosic Heteroxylan Isolated from Alfalfa (Medicago sativa L.) Stem and Its Thermal and Anti-inflammatory Properties

作者：Chen, Lei; Liu, Jie; Zhang, Yaqiong; Niu, Yuge; Dai, Bona; Yu, Liangli^*

来源：Journal of Agricultural and Food Chemistry, 2015, 63(11): 2970-2978.

DOI：10.1021/acs.jafc.5b00063

• alfalfa polysaccharide
• hemicellulosic heteroxylan
• structural analysis
• thermal decomposition
• anti-inflammatory effect

A novel hemicellulosic polysaccharide (ACAP) was purified from the cold alkali extraction of alfalfa stems and characterized as a heteroxylan with a weight-average molecular weight of 7.94 x 10(3) kDa and a radius of 58 nm. Structural analysis indicated that ACAP consisted of a 1,4-linked beta-d-Xylp backbone with 4-O-MeGlcpA and T-l-Araf substitutions at O-2 and O-3 positions, respectively. Transmission electron microscopy (TEM) examination revealed the entangled chain morphology of ACAP molecules. The evaluation of thermal degradation property revealed a primary decomposition temperature range of 238.8-314.0 degrees C with an apparent activation energy (E-a) and a pre-exponential factor (A) of 220.0 kJ/mol and 2.81 x 10(24)/s, respectively. ACAP also showed significant inhibitory activities on IL-1 beta, IL-6, and COX-2 gene expressions in cultured RAW 264.7 mouse macrophage cells. These results suggested the potential utilization of ACAP in functional foods and dietary supplement products.

• 出版日期2015-3-25
• 单位上海交通大学