Authentication of the Oriental Medicinal Plant Ligusticum tenuissimum (Nakai) Kitagawa (Korean Go-Bon) by Multiplex PCR

作者：Jigden Baigalmaa; Wang Hongtao; Kim Myung Kyum; Kim Yeon Ju; In Jun Gyo; Yang Deok Chun^*

来源：Planta Medica, 2010, 76(6): 648-651.

DOI：10.1055/s-0029-1240632

• internal transcribed sequence ( ITS)
• Apiaceae
• Ligusticum tenuissimum
• Ligusticum jeholense
• multiplex PCR
• single nucleotide polymorphism (SNP)

The oriental medicinal plant Ligusticum tenuissimum (Korean name, Go-Bon) is widely used in Korea and China. L. tenuissimum (Go-Bon) has been employed in the treatment of headache and common cold, and as a fever remedy. The internal transcribed spacer (ITS) region was sequenced from thirty-four Go-Bon samples collected from botanical gardens and markets in Korea and China to identify and authenticate L. tenuissimum. Based on the ITS sequences, the thirty-four Go-Bon samples were classified into three groups: L. tenuissimum (Korean Go-Bon), L. jeholense (Chinese GoBon), and unknown Chinese Ligusticum species. Three specific primers were designed to identify the three groups of Ligusticum species using multiplex PCR. The established multiplex-PCR was proved to be effective for the differentiation of L. tenuissimum in commercial plant materials.

• 出版日期2010-4