Dietary vitamin A requirement of Sillago sihama Forskal

作者:Huang, Qin-cheng; Kwaku, Amoah; Du, Tao; Tan, Bei-ping; Chi, Shu-yan; Yang, Qi-hui; Zhang, Shuang; Liu, Hong-yu; Dong, Xiao-hui*; Yang, Yuan-zhi
来源:Aquaculture Research, 2018, 49(7): 2587-2596.
DOI:10.1111/are.13725

摘要

A 56-day growth trial was performed to dietary vitamin A (VA) requirement of Sillago sihama Forsskal (S.sihama) with an initial weight of 2.05 +/- 0.15g (mean +/- SE). Quadruplicate groups of 40 juveniles per tank were fed with diets containing 362, 751, 1,141, 2,202, 4,335, 8,230, and 18,932IUVA/kg diet respectively. Haemorrhages, erosion, greater mortality, and sluggish movement were found in fish of the control, whereas fish fed the 18,932IUVA/kg diet appeared poor appetite. The weight gain rate (WG), specific growth rate (SGR) were highest at the level of 2,202IUVA/kg diet, and the feed conversion ratio (FCR) showed the minimum at the same level. Compared with the control, groups 1,141-18,932IUVA/kg showed significantly higher hepatic lysozyme (LZM) activity. Superoxide dismutase (SOD) at level of 751-4,335IUVA/kg increased significantly than it in the control and reached a plateau after 4,335IUVA/kg. Alkaline phosphatase (AKP) decreased significantly at level of 8,230-18,932IUVA/kg diet than it in the control. Total antioxidation capability (T-AOC), catalase (CAT), and glutathione peroxidase (GSH-Px) in liver tended to increase and kept stable at level of 8,230 and 18,932IUVA/kg diet. Fish fed 2,202IUVA/kg diet showed significantly higher intestinal Na+-K+ ATPase activity than it in other groups. The broken-line model based on WG and maximum liver VA concentration indicated that the VA requirement was of 2,516 and 4,334IUVA/kg respectively.