An investigation on the genetic background of Nostoc flagelliforme by similarity analysis of its partial genomic DNA and phylogenetic comparison of deduced related species

作者：Gao, Xiang^*; Liu, Ke; Qiu, Bao-Sheng

来源：Acta Physiologiae Plantarum, 2011, 33(4): 1301-1318.

DOI：10.1007/s11738-010-0662-z

• Nostoc flagelliforme
• Genetic background
• Genomic DNA
• Phylogenetic comparison

Nostoc flagelliforme, which is distributed on arid and semi-arid steppes of northwestern parts of China, has attracted increasing interest for its stress tolerance. In order to gain more insight into the genetic background of N. flagelliforme, we sequenced its partial genomic DNA for similarity analyses against current public databases, followed by phylogenetic comparison of N. flagelliforme and the potentially related species deduced from the similarity analyses. Approximately 430 kb genomic sequence (similar to 5% of genome as a rough estimate) was determined from 106 distinct genomic clones. Nucleotide BLAST showed that similar to 23.1% of the partial genomic sequence was similar to N. punctiforme genomic DNA and similar to 12.4% to its plasmid DNA. Similar protein search by online FASTA-protein program showed 46.2% of the similar proteins had their corresponding orthologs in N. punctiforme genome. Furthermore, phylogenetic comparison based on 16S rRNA sequences showed N. flagelliforme and N. punctiforme clustered closer among the deduced related species. These results indicated that N. punctiforme might also be potentially close neighbor species of N. flagelliforme, in addition to the formerly regarded close neighbor species N. commune and N. sphaeroids. In general, these data enriched our recognition of the evolutionary relationship between N. flagelliforme and other Nostoc species, especially N. punctiforme.

• 出版日期2011-7
• 单位华中师范大学