Activating killer cell immunoglobulin-like receptors 3DS1 and 2DS1 protect against developing the severe form of recurrent respiratory papillomatosis

作者：Bonagura Vincent R^*; Du Zeying; Ashouri Elham; Luo Lihui; Hatam Lynda J; DeVoti James A; Rosenthal David W; Steinberg Bettie M; Abramson Allan L; Gjertson David W; Reed Elaine F; Rajalingam Raja

来源：Human Immunology, 2010, 71(2): 212-219.

DOI：10.1016/j.humimm.2009.10.009

• Killer cell immunoglobulin-like receptors
• Natural killer cells
• Recurrent respiratory papillomatosis
• Human papillomaviruses-6 and-11
• HLA association

The polymorphic killer cell immunoglobulin-like receptors (KIR) control natural killer (NK) cell response against viral infection and tumor transformation. Here we investigated if select KIR genes are associated with recurrent respiratory papillomatosis (RRP), a rare disease of the larynx and upper airway caused by human papillomaviruses (HPV)-6/11. DNA from 66 RRP patients and 195 healthy controls were characterized for KIR and HLA gene polymorphism. Patients lacking activating KIR genes 3DS1 and 2DS1 were more common in severe RRP compared with mild-moderate RRP (78.8% vs 48.5%, p = 0.019). Further, patients carrying any of the known Susceptible HLA-DRB1/DQB1 alleles were more frequently negative for KIR3DS1 (p = 0.006), KIR2DS1 (p = 0.003) or KIR2DS5 (p = 0.004) compared with controls carrying any of these HLA allotypes. Nearly 80% of the patients with severe disease were missing the protective HLA-DQB1*0602 allele as well as both KIR3DS1 and KIR2DS1 genes. Phenotyping of papilloma-infiltrating mononuclear-cells revealed an elevated numbers of NK cells and CD57(+)CD4(+) T cells in KIR3DS1(-)KIR2DS1(-) patients compared with patients carrying either one or both of these KIRs. Our data suggest that NK cells expressing activating receptors KIR3DS1 and KIR2DS1 may be necessary to trigger an effective early immune response against HPV-infected targets to establish resistance to RRP development. Published by Elsevier Inc. on behalf of American Society for Histocompatibility and Immunogenetics.

• 出版日期2010-2
• 单位河北医科大学