Acetylcholinesterase biosensor based on single-walled carbon nanotubes-Co phtalocyanine for organophosphorus pesticides detection

作者：Ivanov A N; Younusov R R; Evtugyn G A^*; Arduini F; Moscone D; Palleschi G

来源：Talanta, 2011, 85(1): 216-221.

DOI：10.1016/j.talanta.2011.03.045

• Acetylcholinesterase
• Biosensor
• Screen-printed electrode
• Inhibitor measurement

A simple and reliable technique has been developed for the construction of an amperometric acetylcholinesterase biosensor based on screen-printed carbon electrodes. For the first time, one-step modification using single-walled carbon nanotubes and Co phtalocyanine has been proposed to decrease the working potential and to increase the signal of thiocholine oxidation. The biosensor developed made it possible to detect 5-50 ppb of paraoxon and 2-50 ppb of malaoxon with detection limits of 3 and 2 ppb, respectively (incubation 15 min). The biosensor showed high reproducibility when measurements of the substrate and inhibitor were performed (R.S.D. about 1% and 2.5%, respectively). The reliability of the inhibition measurements was confirmed by testing spiked samples of sparkling and tape waters.

• 出版日期2011-7-15