Accurate measurement of optical purity of 2-chloropropionic acid by precolumn derivatization-high performance liquid chromatography and its application

作者：Li Jun Feng; Du Ting Ting; Lan Xian Qiu; Zhang Yi Wen; Song Hang^*

来源：Chinese Journal of Analytical Chemistry, 2007, 35(8): 1195-1198.

• 2-chloropropionic acid
• precolumn derivatization
• chiral stationary phase
• high performance liquid chromatogramphy
• optical purity

2 -Chloropropionic acid was preliminarily derivatized with I -naphthylamine at 30 degrees C for 10 minutes, and then could be satisfactorily separated in 8 min by Chiralcel OD-H column and hexane-alcohol eluent. The effects of composition of mobile phase and column temperature on the chiral separation were investigated. The optimal analysis conditions were obtained as: mobile phase, hexane -methanol -ethanol (50:45:5, V/V); flow rate, 1.0 mL/min; temperature, 30 degrees C; wavelength, 224 nm. More accurate standard specific rotation of 2-chloropropionic acid and relative e. e. values are [alpha](D)(20)-13.94 (neat, dl. 2547) and e. e. 97.9%. This method is proved to be sensitive, easy for operation and good for accurate optical purity measurement of 2-chloropropionic acid.

• 出版日期2007-8
• 单位四川大学