Annealing properties of lyoprotectant solution

作者：Zuo JG; Hua ZZ^*; Liu BL; Zhou GY; Xu Y

来源：Acta Physico - Chimica Sinica, 2005, 21(10): 1178-1181.

• freeze-drying
• tertiary butyl alcohol
• sucrose
• annealing
• differential scanning calorimetry
• glass transition
• devitrification

In order to examine the annealing behaviors of aqueous solutions of 10% tertiary butyl alcohol (TBA) and 10% sucrose in freeze-drying, a differential scanning calorimeter (DSC) has been employed to study freezing properties, annealing temperature, and annealing time. The experimental results show that when the solution is cooled, the existence of sucrose hinders the crystallization of TBA and the value of T-g'; decreases from -32.5 degrees C to -42.0 degrees C, and TBA devitrifies at -30 degrees C when the solution is heated. When annealed at different temperatures around the devitrification peak, TBA all can crystallize completely and the value of T-g'; increases from -42.0 degrees C to -34.9 degrees C. Annealing time depends on annealing temperature. The closer the annealing temperature is to T-g';, the longer the annealing time is. The devitrification will disappear after annealing at -37 degrees C for 20 min.

• 出版日期2005-10
• 单位上海理工大学