Age, serum 25-hydroxyvitamin D and vitamin D receptor (VDR) expression and function in peripheral blood mononuclear cells

作者：Coleman Laura A; Mishina Margarita; Thompson Mark; Spencer Sarah M; Reber Adrian J; Davis William G; Cheng Po Yung; Belongia Edward A; Talbot H Keipp; Sundaram Maria E; Griffin Marie R; Shay David K; Sambhara Suryaprakash

来源：Oncotarget, 2016, 7(24): 35512-35521.

DOI：10.18632/oncotarget.9398

• vitamin D
• vitamin D Receptor
• 1 alpha-hydroxylase
• mRNA expression
• PBMC
• Gerotarget

The relationship between age, vitamin D status, expression and functionality of the vitamin D receptor (VDR), and key genes in the vitamin D pathway in immune cells is unclear. We enrolled adults 50 to 69 years old (20 subjects) and 70+ (20 subjects) and measured: 1) 25(OH)D levels by liquid chromatography/mass spectrometry; and 2) mRNA expression of VDR, 1 alpha-OHase, 1,25D(3)-MARRS, TREM-1, cathelicidin, RIG-I, and interferon-beta by qRT-PCR. Mean serum 25(OH)D was 30 +/- 4 ng/mL and was not associated with age. Baseline expression of VDR, 1 alpha-OHase, 1,25D(3)-MARRS, TREM-1, and RIG-I also did not differ by age; IFN-beta expression, however, was higher in the 70(+) year old group. 25(OH)D-3- and 1,25(OH)(2)D-3-induced VDR, TREM-1 and cathelicidin expression were similar between age groups, as was LPS-induced expression of VDR and of 1 alpha-OHase. Ligand-induced 1,25D(3)-MARRS expression was higher in subjects > 70 years. Serum 25(OH)D was inversely associated with LPS-stimulated VDR expression and with baseline or vitamin D-induced TREM-1 expression, adjusting for age, self-rated health, and functional status. In healthy adults >= 50 years, the expression and functionality of the VDR, 1 alpha-OHase and key vitamin D pathway genes were not consistently associated with age.