Assessment of the LED phototherapy on femoral bone defects of ovariectomized rats: a Raman spectral study

作者：dos Santos Aciole Jouber Mateus; Vieira de Castro Isabele Cardoso; Pinheiro Soares Luiz Guilherme; Santos Barbosa Artur Felipe; dos Santos Aciole Gilberth Tadeu; Silveira Landulfo Jr; Pinheiro Antonio L B^*

来源：Lasers in Medical Science, 2014, 29(3): 1269-1277.

DOI：10.1007/s10103-013-1509-9

• Bone repair
• Light emitting diode
• Osteoporosis

Osteoporosis is a disease characterized by the reduction of bone mineral density. LED wavelengths seem to have similar photo-stimulating effects to laser light. The aim of this study was to assess the Raman shifts: similar to 960 (phosphate hydroxyapatite), similar to 1,070 (carbonate hydroxyapatite), and similar to 1,454 cm (-1) (lipids and proteins) on bone defects of ovariectomized rats treated or not with LED phototherapy (LED-PT). Thirty female rats were divided into four groups (Basal, OVX, OVX+Clot, and OVX+Clot+LED), then subdivided into two subgroups (15 and 30 days after surgery). Osteoporosis induction by ovariectomy (OVX) was performed in all groups, except for the normal basal group. Following development of osteoporosis, one surgical bone defect (5 mm(2)) was created on the femur of each animal. Defects were irradiated with LED light (lambda = 850 +/- 10 nm, P = 150 mW, CW, Dcurrency sign = 0.5 cm(2), 20.4 J/cm(2) per session, t = 128 s, 163.2 J/cm(2) per treatment) at 48 h interval during 2 weeks. Raman measurements were taken at the surface of the defects 30 days after surgery. Significant difference between groups Basal, OVX+Clot, and OVX+Clot+LED for the peaks at similar to 960 (p 0.001; 15 and 30 days), similar to 1,070 (p <= 0.001; 15 and 30 days), and similar to 1,450 cm(-1) (p = 0.002; 15 days; p = 0.004; 30 days) were detected. In addition, statistical differences were obtained between groups OVX, OVX+Clot, and OVX+Clot+LED for these same peaks at all time points (p <= 0.001). At 15 and 30 days, there were statistical differences between groups OVX+Clot and OVX+Clot+LED for the peaks at similar to 960 (p 0.001), similar to 1,070 (p <= 0.001; p = 0.003), and similar to 1,450 cm(-1) (p <= 0.001; p = 0.002). The results of this study are indicative that infrared LED-PT improved the deposition of HA on bone defects of ovariectomized rats.

• 出版日期2014-5