A dual-channel probe with green and near-infrared fluorescence changes for in vitro and in vivo detection of peroxynitrite

作者：Feng, Shumin; Liu, Dandan; Feng, Guoqiang^*

来源：Analytica Chimica Acta, 2019, 1054: 137-144.

DOI：10.1016/j.aca.2018.12.021

• Dual-channel
• Fluorescent probe
• Peroxynitrite
• Bioimaging
• In vitro and in vivo

In this study, a coumarin-semirhodamine hybrid based dual-channel fluorescent probe was developed for detection of peroxynitrite. This probe works well in nearly pure aqueous solution and displays a rapid, highly sensitive and selective response for peroxynitrite at two different emission wavelengths (700 and 515 nm, respectively), along with distinct color and green to near-infrared fluorescence changes. The fluorescent intensity ratio at 700 and 515 nm increases linearly with the concentration of peroxynitrite from 0 to 50 mu M, and the detection limit is estimated to be 59 nM. Moreover, bioimaging of exogenous and endogenous peroxynitrite in living cells and in vivo detection of peroxynitrite with this probe at dual fluorescence channels were all successfully applied. The results suggest that this new probe can be used as a useful imaging tool for studying peroxynitrite in vitro and in vivo.

• 出版日期2019-4-25
• 单位华中师范大学