Methylation Status of CYP27B1 and IGF2 Correlate to BMI SDS in Children with Obesity

作者：Acs Orsolya^*; Peterfia Balint; Hollosi Peter; Luczay Andrea; Torok Dora; Szabo Andras

来源：Obesity Facts, 2017, 10(4): 353-362.

DOI：10.1159/000477462

• DNA methylation
• Childhood obesity
• Insulin-like growth factor 2
• Vitamin D

Objective: Worldwide increasing childhood obesity is due to interactions between environmental and genetic factors, linked together by epigenetic mechanisms such as DNA methylation. Methods: 82 obese children (> 95th BMI percentile, age: 3-18 years) were included. Anthropometric data, metabolic parameters, 25-OH vitamin D (25OHD), and pubertal status were recorded, 24-hour blood pressure monitoring was performed. BMI standard deviation score (SDS) was calculated. Using candidate gene approach, obesity-(insulin-like growth factor 2 (IGF2), proopiomelanocortin (POMC)) and vitamin D metabolism-related genes (1-alfahydroxylase (CYP27B1), VDR) regulated by DNA methylation were selected. After isolating DNA from peripheral blood, bisulfite conversion, bisulfite specific polymerase chain reaction (BS-PCR), and pyrosequencing were carried out. Results: No significant correlation between 25-OHD and metabolic parameters and DNA methylation status, but a tendency of positive correlation between VDR methylation status and 25-OHD (r = 0.2053, p = 0.066) were observed. Significant positive correlations between BMI SDS and CYP27B1 hypermethylation (r = 0.2371, p = 0.0342) and a significant negative correlation between IGF2 hypomethylation and BMI SDS (r = -0.305, p = 0.0059) were found. Conclusions Rate of obesity shows correlation with DNA methylation. Hypomethylation of IGF2 and hypermethylation of CYP27B1 genes might positively influence the rate of BMI observed in obese children.

• 出版日期2017-8