Development of a nano-particle-assisted PCR assay for detection of duck tembusu virus

作者：Wanzhe, Y.; Jianuan, L.; Peng, L.; Jiguo, S.; Ligong, C.; Juxiang, L.

来源：Letters in Applied Microbiology, 2016, 62(1): 63-67.

DOI：10.1111/lam.12509

• detection
• development
• duck tembusu virus
• nano-PCR
• PCR
• sensitivity

Duck tembusu virus (DTMUV) has caused significant economic losses to the poultry industry in China since the spring of 2010. In this study, a nano-PCR assay targeting E gene of DTMUV was developed and their sensitivities and specificities were investigated. Under the optimized conditions of nano-PCR assay for detection of DTMUV, the nano-PCR assay was 10-fold more sensitive than a conventional PCR assay. The lower detection limit of the nano-PCR assay was 1.8 x 10(2) copies mu l(-1) of DTMUV RNA, as no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nano-PCR assay for the detection of DTMUV. The sensitive, and specific nano-PCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of DTMUV-infection.

• 出版日期2016-1
• 单位河北农业大学; 中国动物疫病预防控制中心