Activation of M1 macrophages plays a critical role in the initiation of acute lung injury

作者：Lu, Hui-Lun; Huang, Xin-Yan; Luo, Yi-Feng; Tan, Wei-Ping; Chen, Pei-Fen; Guo, Yu-Biao^*

来源：Bioscience Reports, 2018, 38(2): BSR20171555.

DOI：10.1042/BSR20171555

• RESPIRATORY-DISTRESS-SYNDROME
• ISCHEMIA-REPERFUSION INJURY
• NF-KAPPA-B
• POLARIZATION
• INFLAMMATION
• INHIBITION
• RESOLUTION
• LIPOPOLYSACCHARIDE
• POPULATIONS
• DEPLETION

The goal of the present study was to investigate the role of M1 macrophages in acute lung injury (ALI). To address this, we used lipopolysaccharide (LPS)-treated wild-type and CD11b-DTR mice, and examined their M1 macrophage levels, and the extent of their inflammation and pulmonary injuries. In addition, we evaluated pulmonary function by measuring the expressions of SP-A and SP-B in infiltrated M1 macrophages. Finally, we co-cultured the mouse type II-like alveolar epithelial cells (AT-II) and mouse pulmonary microvascular endothelial cells (PMECs) with M1 macrophages in the presence of TNF-alpha or H2O2 and assessed them for viability and apoptosis. After LPS treatment, we observed that the number of pulmonary M1/M2 macrophages and the serum levels of interleukin-1 beta (IL-1 beta), tumor necrosis factor a (TNF-alpha), and reactive oxygen species (ROS) significantly increased. Furthermore, the increase in cytokines was accompanied with the initiation of lung injury indicated by the decreased levels of SP-A and SP-B. In macrophage-depleted CD11b-DTR mice, ALI was attenuated, serum levels of IL-1 beta, TNF-alpha and ROS were reduced, and lung levels of monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) were decreased. After administering TNF-alpha and H2O2, the proapoptotic effect of M1 macrophages on AT-II or PMECs significantly increased, the cell viabilities significantly decreased, and apoptosis significantly increased. Our results suggest that M1 macrophages are recruited to the lungs where they significantly contribute to an increase in TNF-alpha and ROS production, thus initiating ALI.

• 出版日期2018-4-27
• 单位中山大学