High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins

作者：Zawadzka Kazimierczuk Anna; Kozminski Wiktor^*; Sanderova Hana; Krasny Libor

来源：Journal of Biomolecular NMR, 2012, 52(4): 329-337.

DOI：10.1007/s10858-012-9613-x

• Intrinsically disordered proteins
• Non-uniform sampling
• Backbone assignment
• 5D NMR
• 6D NMR

Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences.

• 出版日期2012-4