A C-type lectin with an immunoglobulin-like domain promotes phagocytosis of hemocytes in crayfish Procambarus clarkii

作者：Zhang, Xiao-Wen; Wang, Yue; Wang, Xian-Wei^*; Wang, Lei; Mu, Yi; Wang, Jin-Xing^*

来源：Scientific Reports, 2016, 6(1): 29924.

DOI：10.1038/srep29924

• SHRIMP
• GENE
• DIVERSITY
• PROTEINS
• FAMILY
• HEPATOPANCREAS
• SUPERFAMILY
• MOLECULES
• DSCAM

C-type lectins are important immune molecules that participate in host defense response. The present work reports a novel C-type lectin (PcLec3) from the red swamp crayfish Procambarus clarkii. Sequence analysis found that PcLec3 encodes a polypeptide with252 amino acid residues, which contains an immunoglobulin-like domain (IG) and a C-type lectin domain (CTLD) arranged in tandem. Tissue distribution analysis indicated that PcLec3 is enriched expressed in hemocytes and hepatopancreas cells, in which PcLec3 was up-regulated following bacterial challenge by Vibrio anguillarum. Function analysis using recombinant full-length PcLec3, IG, and CTLD proteins revealed that these recombinant proteins had the capacity to bind carbohydrates and bacteria, while IG determined the cell binding activity. However, only full-length PcLec3 promotes the phagocytic activity of hemocytes and subsequent clearance of invasive bacteria. Taken together, these results manifest that PcLec3 acts as a hemocyte adhesion molecule to promote hemocyte phagocytosis against invasive V. anguillarum.

• 出版日期2016-7-14
• 单位河南师范大学; 山东大学