EPs763((R)) from Pelargonium sidoides increases stress resistance in Caenorhabditis elegans probably via the DAF-16/FOXO pathway

作者：Rezaizadehnajafi Leila; Wink Michael^*

来源：Phytomedicine, 2014, 21(4): 547-550.

DOI：10.1016/j.phymed.2013.10.027

• Pelargonium sidoides
• Caenorhabditis elegans
• Stress resistance
• DAF-16

EPs7630((R)) a water alcohol extract of the roots from Pelargonium sidoides contains several secondary metabolites including highly oxygenated coumarins, various phenolics and polyphenols. Using the DPPH center dot assay to measure antioxidant activity a free radical scavenging activity of 14.7 +/- 0.85 mu g/ml (IC50) was determined. As an in vivo model Caenorhabditis elegans was applied to study the effect of EPs7630((R)) on stress resistance. EPs7630((R)) treatment reduces intracellular hsp-16.2::GFP expression (induced by the pro-oxidant juglone) indicating that the secondary metabolites of EPs7630((R)) are bioavailable and exhibit antioxidant activities in vivo. Application of EPs7630((R)) (50 mu g/ml) to the transgenic mutant TJ356 induced the migration of the transcription factor DAF-16 from cytosol to the nucleus, suggesting a prominent role of DAF-16/FOXO in the daf-2 pathway for stress resistance.

• 出版日期2014-3-15