PAF-METABOLIC ENZYMES AND PAF-LIKE ACTIVITY IN L. INFANTUM AND L. MAJOR PROMASTIGOTES

作者：Chatzovoulos P; Tsoupras A B^*; Samiotaki M; Panayotou G; Demopoulos C A; Dotsika E

来源：European Journal of Inflammation, 2011, 9(3): 231-239.

• Platelet Activating Factor (PAF)
• PAF-CPT
• Lyso-PAF-AT
• inflammation
• Leishmania major
• Leishmania infantum
• leismaniasis
• infection

Leishmania spp are obligate intracellular protozoan parasites of the mononuclear phagocyte system that cause a spectrum of diseases known as leishmaniasis. Platelet Activating Factor (PAF) and some PAF-antagonists seem to play a key role in Leishmania infection. In this article we detected for the first time the specific activities of PAF basic metabolic enzymes, PAF cholinephosphotransferase (PAF-CPT) and Lyso-PAF-acetylotransferase (Lyso-PAF-AT), in two species of Leishmania, namely Leishmania major (L. major) and Leishmania infantum (L. infantum). Specific activity of PAF-CPT of L. major homogenates was substantially higher than that of L. infantum homogenates, while Lyso-PAF-AT specific activities of the homogenates of both species were detected in the same levels. In addition, PAF-like molecules were detected in cells and their culture medium of both species. These results show that PAF-biosynthesis exists and varies between Leishmania species, findings that are in correlation with the general idea that the presence of PAF in several Leishmania species is implicated in Leishmania infection.

• 出版日期2011-12