Does Dexamethasone Inhibit the Antineoplastic Effect of Cisplatin and Docetaxel in Head and Neck Cancer Cells?

作者：Wagenblast Jens^*; Arnoldner Christoph; Gstoettner Wolfgang; Bisdas Sotirios; Moertel Susanne; May Angelika; Hambek Markus

来源：Anticancer Research, 2010, 30(1): 123-127.

• Cisplatin
• docetaxel
• dexamethasone
• squamous cell carcinoma of the head and neck (SCCHN) cell lines
• resistance
• apoptosis

Background/Aim: Comedication with glucocorticoids such as dexamethasone is frequently given to head and neck cancer patients treated with chemotherapy. However, all increasing body of evidence suggests that dexamethasone may induce resistance to antineoplastic agents. The present study Was the first to investigate the effect of dexamethasone oil the antiproliferative activity of cisplatin and docetaxel in vitro in squamous cell carcinoma of the head and neck (SCCHN) cell lines. Materials and Methods: The cytotoxic effect of cisplatin and docetaxel oil eight SCCHN cell lines was determined for each drug alone or with increasing concentrations of dexamethasone. Cell growth inhibition and viability were measured quantitatively after 24, 48, 72 hours of treatment using water-soluble-tetrazolium-test and lactate dehydrogenase assays. Absolute tumor cell numbers were determined by cell counting in a Rosenthal chamber Results: Cisplatin and docetaxel alone inhibited the growth of all eight SCCHN cell lines significantly (p=0.012). The antiproliferative activity of both agents was not decreased by the addition of dexamethasone in any of the cell lines (p>0.05). Conclusion: Dexamethasone does not interfere with the cytotoxic action of cisplatin or docetaxel in the investigated SCCHN cell lines.

• 出版日期2010-1