Design of a single-step immunoassay principle based on the combination of an enzyme-labeled antibody release coating and a hydrogel copolymerized with a fluorescent enzyme substrate in a microfluidic capillary device

作者：Wakayama Hideki; Henares Terence G; Jigawa Kaede; Funano Shun ichi; Sueyoshi Kenji; Endo Tatsuro; Hisamoto Hideaki^*

来源：Lab on a Chip, 2013, 13(22): 4304-4307.

DOI：10.1039/c3lc50775k

A combination of an enzyme-labeled antibody release coating and a novel fluorescent enzyme substrate-copolymerized hydrogel in a microchannel for a single-step, no-wash microfluidic immunoassay is demonstrated. This hydrogel discriminates the free enzyme-conjugated antibody from an antigen-enzyme-conjugated antibody immunocomplex based on the difference in molecular size. A selective and sensitive immunoassay, with 10-1000 ng mL(-1) linear range, is reported.

• 出版日期2013