Burkholderia pseudomallei induces IL-23 production in primary human monocytes

作者：Kulsantiwong Panthong; Pudla Matsayapan; Boondit Jitrada; Wikraiphat Chanthiwa; Dunachie Susanna J; Chantratita Narisara; Utaisincharoen Pongsak^*

来源：Medical Microbiology and Immunology, 2016, 205(3): 255-260.

DOI：10.1007/s00430-015-0440-z

• B. pseudomallei
• Human monocytes
• IL-23
• Melioidosis

Burkholderia pseudomallei, a gram-negative intracellular bacterium, is a causative agent of melioidosis. The bacterium has been shown to induce the innate immune response, particularly pro-inflammatory cytokine production in several of both mouse and human cell types. In the present study, we investigate host immune response in B. pseudomallei-infected primary human monocytes. We discover that wild-type B. pseudomallei is able to survive and multiply inside the primary human monocytes. In contrast, B. pseudomallei LPS mutant, a less virulent strain, is susceptible to host killing during bacterial infection. Moreover, microarray result showed that wild-type B. pseudomallei but not B. pseudomallei LPS mutant is able to activate gene expression of IL-23 as demonstrated by the up-regulation of p19 and p40 subunit expression. Consistent with gene expression analysis, the secretion of IL-23 analyzed by ELISA also showed that wild-type B. pseudomallei induces a significantly higher level of IL-23 secretion than that of B. pseudomallei LPS mutant. These results implied that IL-23 may be an important cytokine for the innate immune response during B. pseudomallei infection. The regulation of IL-23 production may drive the different host innate immune responses between patients and may relate to the severity of melioidosis.

• 出版日期2016-6