科研之友
微信
新浪微博
Facebook
分享链接
摘要
Background. Positron emission tomography (PET) has the potential to be a specific, sensitive and quantitative diagnostic test for transplant rejection. To test this hypothesis, we evaluated F-18-labeled fluorodeoxyglucose ([F-18]FDG) and 13N-labeled ammonia ([N-13]NH3) small animal PET imaging in a well-established murine cardiac rejection model. Methods. Heterotopic transplants were performed using minor major histocompatibility complex-mismatched B6.C-H2(bm12) donor hearts in C57BL/6(H-2(b)) recipients. C57BL/6 donor hearts into C57BL/6 recipients served as isograft controls. [F-18]FDG PET imaging was performed weekly between posttransplant days 7 and 42, and the percent injected dose was computed for each graft. [N-13]NH3 imaging was performed to evaluate myocardial perfusion. Results. There was a significant increase in [F-18]FDG uptake in allografts from day 14 to day 21 (1.6% to 5.2%; P < 0.001) and uptake in allografts was significantly increased on posttransplant days 21 (5.2% vs 0.9%; P = 0.005) and 28 (4.8% vs 0.9%; P = 0.006) compared to isograft controls. Furthermore, [F-18]FDG uptake correlated with an increase in rejection grade within allografts between days 14 and 28 after transplantation. Finally, the uptake of [N-13]NH3 was significantly lower relative to the native heart in allografts with chronic vasculopathy compared to isograft controls on day 28 (P = 0.01). Conclusions. PET imaging with [F-18]FDG can be used after transplantation to monitor the evolution of rejection. Decreased uptake of [N-13]NH3 in rejecting allografts may be reflective of decreased myocardial blood flow. These data suggest that combined [F-18]FDG and [N-13]NH3 PET imaging could be used as a noninvasive, quantitative technique for serial monitoring of allograft rejection and has potential application in human transplant recipients.
- 出版日期2015-9
