ALPHA-1 SUBUNITS OF VOLTAGE-GATED CA2+ CHANNELS IN THE MESENCEPHALON X NEUROBLASTOMA HYBRID CELL-LINE MES23.5

作者：SCHNEIDER T; PEREZREYES E; NYORMOI O; WEI X; CRAWFORD GD; SMITH RG; APPEL SH; BIRNBAUMER L

来源：Neuroscience, 1995, 68(2): 479-485.

DOI：10.1016/0306-4522(95)00147-B

The identity of alpha(1) subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon x N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(alpha(1D)), Q-(alpha(1A)) and omega-conotoxin GVIA sensitive N-type (alpha(1B)) Ca2+ channel alpha(1) subunits. The amplified fragments, which are homologous to the region between domain III and IV of known alpha(1) subunits, reveal splice variation in the L- and Q-type alpha(1) subunit of MES23.5 cells. The transcripts of alpha(1) subunits in these cells were quantified by RNAase protection assay.
The data show the existence of different Ca2+ channel types in a single cell line and may reflect multiple functions of voltage operated Ca2+ channels during growth, differentiation and transmitter release.

• 出版日期1995-9