A Nanopore-Nanofiber Mesh Biosensor To Control DNA Translocation

作者：Squires Allison H; Hersey Joseph S; Grinstaff Mark W^*; Meller Amit

来源：Journal of the American Chemical Society, 2013, 135(44): 16304-16307.

DOI：10.1021/ja408685x

Solid-state nanopores show promise as single-molecule sensors for biomedical applications, but to increase their resolution and efficiency, analyte molecules must remain longer in the nanopore sensing volume. Here we demonstrate a novel, facile, and customizable nanopore sensor modification that reduces the double-stranded DNA translocation velocity by 2 orders of magnitude or more via interactions outside the nanopore. This is achieved by electrospinning a copolymer nanofiber mesh (NFM) directly onto a solid-state nanopore (NP) chip. The effect of NFMs on dsDNA translocation through an NP is highlighted using a set of NFMs of varying mesh composition that reduce the translocation speed relative to a bare pore from 1- to %26gt;100-fold. A representative NFM from this set is effective on DNA as long as 20 kbp, improves the nanopore resolution, and allows discrimination among different DNA lengths.

• 出版日期2013-11-6