Brain-Derived Microparticles in Patients with Severe Isolated TBI

作者：Nekludov M^*; Bellander B M; Gryth D; Wallen H; Mobarrez F

来源：Brain Injury, 2017, 31(13-14): 1856-1862.

DOI：10.1080/02699052.2017.1358395

• traumatic brain injury
• microparticles
• clinical study
• cerebrovenous blood

Primary Objective: to Investigate the presence of circulating microparticles (MPs) of brain tissue origin In the systemic and cerebrovenous blood of patients with severe traumatic brain Injury (TBI).& para;& para;Research Design: Prospective observational study in 15 consecutive patients with severe isolated TBI.& para;& para;Methods and Procedures: We repeatedly measured concentrations of MPs expressing glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE) and aquaporin-4 (AQP4), in arterial and cerebrovenous blood at admittance to hospital and up to 72 hours after the injury.& para;& para;Main Outcomes and Results: Concentrations of MPs expressing GFAP and AQP4 were significantly higher In the TBI group compared with healthy controls: GFAP 2.0 [1.1-7.9] vs. 1.3 [1-2.1] x 10(6)/mL, p < 0.001; AQP4 0.1 [0.07-0.22] vs. 0.08 [0.06-0.11] x 10(6)/mL, p < 0.001 (median, range). No transcranial gradients were found. Levels of NSE-expressing MPs were also higher in the TBI group compared with healthy controls: 0.4 [0.25-2.1] vs. 0.26 [0.13-0.98] x 10(6)/mL, p < 0.05; however, regarding NSE-posItive non-platelet MPs, there were no differences between patients and controls.& para;& para;Conclusions: Patients with TBI have higher numbers of brain-derived MPs. Further studies are needed, however, to Identify specific and sensitive MP markers of brain Injury.

• 出版日期2017