Hydrophilic polymeric nanoparticles prepared from Delonix galactomannan with low cytotoxicity for ocular drug delivery

作者：Ogunjimi Abayomi T; Melo Shaiani M G; Vargas Rechia Carem G; Emery Flavio S; Lopez Renata F V^*

来源：Carbohydrate Polymers, 2017, 157: 1065-1075.

DOI：10.1016/j.carbpol.2016.10.076

• Cellular uptake
• Cytotoxicity
• Delonix polymeric nanoparticles
• Ocular delivery
• Quality by design

Delonix is a galactomannan polysaccharide extracted from the endosperm of Delonix regia plant. This study aims at the development of Delonix nanoparticle and assesses its potential for ocular delivery by evaluating its in-vitro stability, toxicity and cellular uptake. Fluorescent nanoparticles (BODIPY-loaded nanoparticles) were prepared by a Quality-by-Design modified nanoprecipitation technique. Optimized nanoparticles had mean sizes <240 nm, PdI <0.2 and zeta potential of <-30 mV. Mixture of surfactants with different hydrophilic-lipophilic balance controlled nanoparticle swelling. Nanoparticles, which were stable in the presence of simulated lachrymal fluid and lysozyme also sustained the release of BODIPY. In-vitro studies suggest no toxicity of the nanoparticles in concentration range of 100-1483.3 mu g/mL on retinal and corneal epithelial cells. Flow cytometry and confocal microscopy techniques showed that retinal cells but not corneal cells, uptake 18% of the nanoparticles. Therefore, Delonix nanoparticles could be a safe and promising tool for ocular drug delivery.

• 出版日期2017-2-10