High-fat diet causes an imbalance in the colonic serotonergic system promoting adipose tissue enlargement and dysplasia in rats

作者：Kannen Vinicius^*; Zanette Dalila L; Fernandes Cleverson R; Ferreira Frederico R; Marini Tassiana; Carvalho Milene C; Brandao Marcus L; Elias Junior Jorge; Mauad Fernando M; Silva Wilson A Jr; Stopper Helga; Garcia Sergio B

来源：Toxicology Letters, 2012, 213(2): 135-141.

DOI：10.1016/j.toxlet.2012.06.014

• High-fat diet
• Serotonergic system
• Colon dysplasia
• Angiogenesis
• Proliferation
• Cyclooxygenase-2

A high-fat (HF) diet, the serotonergic system and stromal elements have all been implicated in colon carcinogenesis. We investigated whether the colonic serotonergic system could play a main role in the development of colonic dysplasia and stromal reactivity in carcinogen-treated rats under HF diet. For this, dimethylhydrazine-treated rats were fed with standard diet and a HF diet. Fat distribution was quantified by computerized tomography exam, serotonergic activity was analyzed by high-performance liquid chromatography, gene expression, and immunohistochemistry, which along with histopathological technique enabled us to enumerate dysplasia, microvessels density, cell proliferation and COX-2 expression. We found that the HF diet induced an increase in the amount of viscera! adipose tissue, even without expressive changes in the average body weight. This was correlated with a loss of serotonergic balance in colon tissue. Moreover, the HF diet promoted dysplasia and microvessel density in association with increased proliferation and COX-2 expression within pericryptal colonic stroma. Our current findings suggest that a HF diet promotes the enlargement of adipose tissue via loss of control in colon serotonergic activity, which enhances colonic dysplasia by supporting microvessel development.

• 出版日期2012-9-3