科研之友
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摘要
A new approach for improving and scaling up the purification of recombinant human interferon gamma (hIFN gamma) expressed in Escherichia coli and accumulated in the form of inclusion bodies was investigated. The new strategy involves denaturation of the inclusion bodies in 7.4M guanidine hydrochloride, loading on a hydrophobic (Octyl-Sepharose) column and elution with urea/ammonium chloride. The method is fast, simple and low in cost. For maximum recovery of the hIFN gamma biological activity, an optimization of the refolding step is proposed.
- 出版日期2009-2
