EXTRACTS OF AMBROSIA ARTEMISLIFOLIA L. AND ITS TOXICITY EFFECTS ON MICE

作者：Bo Zhao; Zhang, Guocai^*; Zhang, Xinqian; Ying Bao; Zhang, Qiushuang

来源：Bangladesh Journal of Botany, 2017, 46(2): 539-548.

• Ambrosia artemisiifolia
• Active extracts
• Ultrasonic/microwave assisted extraction
• Response surface methodology
• Acute toxicity

A new and effective method of ultrasonic/microwave assisted extraction (UMAE) of the active extracts from Ambrosia artemisiifolia L. (AAE) and to investigate its acute toxicity had been proposed. The response surface methodology (RSM) was employed to optimize the UMAE conditions. The optimum conditions were as follows: sonication time, 28.50 min, microwave treatment, 2.40 min, microwave temperature, 70 degrees C and liquor to material ratio, 8.00 (ml/g). Then four obtained extraction methods were compared. The results indicated that the extracts produced by UMAE had the highest yield and the highest anti-pathogenic activity. The acute toxicity of AAE was studied in mice. The LD50 was 4966.33 mg/kg, which belonged to a low level of mammalian toxicity. Furthermore, the AAE could activate the activity of SOD in mice livers, while inhibiting the CAT and POD activities. The results suggested that A. artemisiifolia was a novel antimicrobial agent. It also instigated thoughts of security.

• 出版日期2017-6
• 单位东北林业大学