A new alpha-gliadin gene family for wheat breeding: somatic introgression line II-12 derived from Triticum aestivum and Agropyron elongatum

作者：Chen Fanguo; Xu Chunhui; Chen Mengzhu; Wang Yanhui; Xia Guangmin^*

来源：Molecular Breeding, 2008, 22(4): 675-685.

DOI：10.1007/s11032-008-9208-0

• Triticum aestivum
• Agropyron elongatum
• somatic hybrid introgression line
• origination of novel alpha-gliadin
• wheat breeding

Ninety-five alpha-gliadin open reading frames were cloned and sequenced from the somatic hybrid wheat introgression line II-12 and its parents Triticum aestivum cv. Jinan177 (JN177) and Agropyron elongatum. Novel alpha-gliadin genes were found to originate via point mutation, unequal crossover or slippage of a parental gene, demonstrating that new genes could be rapidly created through somatic hybridization in a manner similar to that previously shown for high-molecular-weight glutenin subunits (HMW-GS) genes. The data reveal the composition and origin of the alpha-gliadin gene in II-12, showing that: (1) most were homologous to those of JN177; (2) a few were derived direct from A. elongatum; and (3) some new genes were created de novo. A particular quality attribute of interest was the presence or absence of celiac disease (CD) epitopes, which were found to be four times more common among alpha-gliadin genes from the parent wheat JN177 than in those from A. elongatum. Although four types of CD epitopes were found in introgression line II-12, the number of genes encoded CD epitopes was lower than in JN177 due to the occurrence of pseudogenes. We discuss the benefit of these alpha-gliadins to wheat breeding.

• 出版日期2008-11
• 单位山东大学