A feasibility study of an integrated NIR/gamma/visible imaging system for endoscopic sentinel lymph node mapping

作者：Kang Han Gyu; Lee Ho Young; Kim Kyeong Min; Song Seong Hyun; Hong Gun Chul; Hong Seong Jong

来源：Medical Physics, 2017, 44(1): 227-239.

DOI：10.1002/mp.12029

• endoscopy
• multimodal imaging
• near-infrared (NIR)
• scintigraphy
• sentinel lymph node (SLN) mapping

Purpose: The aim of this study is to integrate NIR, gamma, and visible imaging tools into a single endoscopic system to overcome the limitation of NIR using gamma imaging and to demonstrate the feasibility of endoscopic NIR/gamma/visible fusion imaging for sentinel lymph node (SLN) mapping with a small animal.
Methods: The endoscopic NIR/gamma/visible imaging system consists of a tungsten pinhole collimator, a plastic focusing lens, a BGO crystal (11 x 11 x 2 mm(3)), a fiber-optic taper (front = 11 x 11 mm(2), end = 4 x 4 mm(2)), a 122-cm long endoscopic fiber bundle, an NIR emission filter, a relay lens, and a CCD camera. A custom-made Derenzo-like phantom filled with a mixture of Tc-99m and indocyanine green (ICG) was used to assess the spatial resolution of the NIR and gamma images. The ICG fluorophore was excited using a light-emitting diode (LED) with an excitation filter (723-758 nm), and the emitted fluorescence photons were detected with an emission filter (780-820 nm) for a duration of 100 ms. Subsequently, the Tc-99m distribution in the phantom was imaged for 3 min. The feasibility of in vivo SLN mapping with a mouse was investigated by injecting a mixture of Tc-99m-antimony sulfur colloid (12 MBq) and ICG (0.1 mL) into the right paw of the mouse (C57/B6) subcutaneously. After one hour, NIR, gamma, and visible images were acquired sequentially. Subsequently, the dissected SLN was imaged in the same way as the in vivo SLN mapping.
Results: The NIR, gamma, and visible images of the Derenzo-like phantom can be obtained with the proposed endoscopic imaging system. The NIR/gamma/visible fusion image of the SLN showed a good correlation among the NIR, gamma, and visible images both for the in vivo and ex vivo imaging.
Conclusion: We demonstrated the feasibility of the integrated NIR/gamma/visible imaging system using a single endoscopic fiber bundle. In future, we plan to investigate miniaturization of the endoscope head and simultaneous NIR/gamma/visible imaging with dichroic mirrors and three CCD cameras.

• 出版日期2017-1