Developmental expression of the Arabidopsis thaliana CycA2;1 gene

作者：Burssens S; Engler JD; Beeckman T; Richard C; Shaul O; Ferreira P; Van Montagu M; Inze D^*

来源：Planta, 2000, 211(5): 623-631.

DOI：10.1007/s004250000333

• Arabidopsis (cell cycle)
• cell cycle
• cyclin
• embryogenesis
• gene expression (cyclin)
• phytohormones

The associations of cyclins with highly conserved cyclin-dependent kinases are key events in the regulation of cell cycle progression. The spatio-temporal expression of an Arabidopsis thaliana (L.) Heynh. mitotic cyclin, Arath;CycA2;1, was studied by histochemical P-glucuronidase (GUS) analysis and in-situ hybridizations. The CycA2;1 promoter was active in the egg apparatus before fertilization. During embryogenesis, CycA2;1:gus expression was found in the embryo and the developing endosperm. Throughout plant development, CycA2;1 transcripts were found in both dividing and non-dividing cells, indicating that the expression of this cyclin is not a limiting factor for cell division. In the pericycle and stelar parenchyma, CycA2, I transcripts were located at the xylem poles, a position that can be correlated with competence for lateral root formation. In addition, CycA2;1:gus expression was upregulated in roots by auxins and in the shoot apex by cytokinins. Transcription of CycA2;1 was shown by reverse transcription-polymerase chain reaction to be strongly induced by sucrose in A. thaliana cell suspensions.

• 出版日期2000-10