An Enzyme-linked Immunosorbent Assay for Detection of Fenoxaprop-p-ethyl

作者：Wang Jun Dong^*; Shi Hai Yan; Ye Yong Hao; Wang Ming Hua

来源：Chinese Journal of Analytical Chemistry, 2009, 37(1): 82-86.

DOI：10.1159/000210413

• Fenoxaprop-p-ethyl
• polyclonal antibody
• enzyme-linked immunosorbent assay

To develop a competitive indirect enzyme-linked immunosorbent assay (ci-ELISA) for fenoxaprop-p-ethyl, a hapten (fenoxaprop acid) was synthesized. Hapten conjugated to bovine serum albumin (BSA) with the carbodiimide method was used as immunogens and ovalbumin (OVA) with mixed anhydride method was used as the coating antigens. The New Zealand rabbits were immunized by conjugate of Hapten-BSA and titres of anti-fenoxaprop-p-ethyl serum (1.024 x 10(5)) were determined by non-competitive indirect enzyme-linked assay procedure. After optimization of the ic-ELISA conditions, such as pH values, inonic strengths, concentrations of methanol, pH 7.5, PBS of 0.3 mol/L Na(+) and 10% methanol were determined as optimum assay conditions. The IC(50) for fenoxaprop-p-ethyl was (0.084 +/- 0.012) mg/L, and lower detection limit (LDL) was (0.0064 +/- 0.002) mg/L. The cross-reactivities of other aryloxyphenoxypropionic acid herbicides to the antibodies were less than 0.1% in the assays, except metamifop because of its structures.

• 出版日期2009-1
• 单位南京农业大学