Short communication: Simultaneous analysis of reducing sugars and 5-hydroxymethyl-2-furaldehyde at a low concentration by high performance anion exchange chromatography with electrochemical detector, compared with HPLC with refractive index detector

作者：Guan, Y-G.; Yu, P.; Yu, S-J.^*; Xu, X-B.; Wu, X-L.

来源：Journal of Dairy Science, 2012, 95(11): 6379-6383.

DOI：10.3168/jds.2012-5623

• high performance anion exchange chromatography with electrochemical detector
• HPLC with refractive index detector
• 5-hydroxymethyl-2-furaldehyde
• reducing sugar

A simultaneous analysis of reducing sugars and 5-hydroxymethyl-2-furaldehyde of the Maillard reaction products was detailed. It was based on a high performance anion exchange chromatography with electrochemical detector system and an HPLC with refractive index detector. Results showed that high performance anion exchange chromatography with electrochemical detector using a CarboPac PA-1 column (Dionex Corp., Sunnyvale, CA) was more suitable for reducing sugars and 5-hydroxymethyl-2-furaldehyde determination, especially for trace analysis. The lowest detectable limit of reducing sugars and 5-hydroxymethyl-2-furaldehyde was 0.00005 mol/L in this experiment. However, HPLC with a refractive index detector always produces a. tailing peak for 5-hydroxymethyl-2-furaldehyde, and mannose and fructose cannot be absolutely separated. The results of the present study could provide a more sensitive means for 5-hydroxymethyl-2-furaldehyde and reducing sugar detection.

• 出版日期2012-11
• 单位华南理工大学