Schisandra sphenanthera Extract Facilitates Liver Regeneration after Partial Hepatectomy in Mice

作者：Li, Xi; Fan, Xiaomei; Li, Dongshun; Zeng, Xuezhen; Zeng, Hang; Wang, Yongtao; Zhou, Yawen; Chen, Yixin; Huang, Min^*; Bi, Huichang^*

来源：Drug Metabolism and Disposition, 2016, 44(5): 647-652.

DOI：10.1124/dmd.115.068288

• ACETAMINOPHEN-INDUCED HEPATOTOXICITY
• HEPATIC RESECTION
• FAILURE
• RAT
• REPLICATION
• MECHANISMS
• TACROLIMUS
• EXPRESSION
• CHINENSIS
• PROTEINS

Liver regeneration after surgical liver resection is crucial for the restoration of liver mass and the recovery of liver function. Schisandra sphenanthera extract (Wuzhi tablet, WZ) is a preparation of an extract from the dried ripe fruit of Schisandra sphenanthera Rehd. et Wils, a traditional hepatoprotective herb. Previously, we found that WZ could induce liver regeneration-related genes against acetaminophen-induced liver injury. However, whether WZ can directly facilitate liver regeneration after liver resection remains unknown. We investigated whether WZ has potential in promoting liver regeneration after a partial hepatectomy (PHX) in mice. Remnant livers were collected 1, 1.5, 2, 3, 5, 7, and 10 days after PHX. Hepatocyte proliferation was assessed using the Ki-67 labeling index. Western blot analysis was performed on proteins known to be involved in liver regeneration. The results demonstrated that WZ significantly increased the liver-to-body weight ratio of mice after PHX but had no effect on that of mice after a sham operation. Additionally, the peak hepatocyte proliferation was observed at 1.5 days in PHX/WZ-treated mice but at 2 days in PHX/saline-treated mice, as evidenced by the Ki-67 positive ratio. Furthermore, WZ significantly increased the protein expression of ligand-induced phosphorylation of epidermal growth factor receptor and up-regulated cyclin D1, cyclin D-dependent kinase 4, phosphorylated retinoblastoma, and proliferating cell nuclear antigen protein expression and down-regulated the expression of cell cycle inhibitors p21 and p27 in the regenerative process after PHX. These results demonstrate that WZ significantly facilitates hepatocyte proliferation and liver regeneration after PHX.

• 出版日期2016-5
• 单位中山大学