Schisandrae Fructus Inhibits IL-1 beta-Induced Matrix Metalloproteinases and Inflammatory Mediators Production in SW1353 Human Chondrocytes by Suppressing NF-kappa B and MAPK Activation

作者：Jeong Jin Woo; Lee Hye Hyeon; Choi Eun Ok; Lee Ki Won; Kim Ki Young; Kim Sung Goo; Hong Su Hyun; Kim Gi Young; Park Cheol; Kim Ho Kyoung; Choi Young Whan; Choi Yung Hyun

来源：Drug Development Research, 2015, 76(8): 474-483.

DOI：10.1002/ddr.21283

• Schisandra chinensis
• osteoarthritis
• matrix metalloproteinase
• NF-kappa B
• mitogen-activated protein kinases

Proinflammatory cytokine interleukin-1 beta (IL-1 beta) plays a crucial role in the pathogenesis of osteoarthritis (OA) by stimulating several mediators that contribute to cartilage degradation. Schisandrae Fructus (SF), the dried fruit of Schisandra chinensis (Turcz.) Baill. (Magnoliaceae), is widely used in traditional medicine for the treatment of a number of chronic inflammatory diseases. This study investigated the antiosteoarthritis properties of an ethanol extract of SF on IL-1 beta-stimulated SW1353 chondrocytes. SF attenuated IL-1 beta-induced expression and activity of matrix metalloproteinase (MMP)-1, MMP-3, and MMP-13 and also reduced the elevated levels of cyclooxygenase-2 and inducible nitric oxide synthase associated with the inhibition of prostaglandin E-2 and nitric oxide production in IL-1 beta-stimulated SW1353 chondrocytes. In addition, SF markedly suppressed the nuclear translocation of nuclear factor-kappa B (NF-kappa B) by blocking inhibitor kappa B-alpha degradation and inhibited the phosphorylation of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK). These results indicate that the inhibitory effect of SF on IL-1 beta-stimulated expression of MMPs and inflammatory mediators production in SW1353 cells were associated with the suppression of the NF-kappa B and JNK/p38 MAPK signaling pathways. The results from this study indicate that SF may have therapeutic potential for the treatment of OA due to its anti-inflammatory and chondroprotective features.

• 出版日期2015-12