Aequorin-expressing yeast emits light under electric control

作者：Vilanova Cristina; Hueso Angeles; Palanca Carles; Marco Guillem; Pitarch Miguel; Otero Eduardo; Crespo Juny; Szablowski Jerzy; Rivera Sara; Dominguez Escriba Laura; Navarro Emilio; Montagud Arnau; Fernandez de Cordoba Pedro; Gonzalez Asier; Arino Joaquin; Moya Andres; Urchueguia Javier; Porcar Manuel^*

来源：Journal of Biotechnology, 2011, 152(3): 93-95.

DOI：10.1016/j.jbiotec.2011.01.005

• Aequorin
• Bioluminescence
• Yeast electro-stimulation
• Synthetic biology
• Bioelectronics

In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5 V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.

• 出版日期2011-3-20