Analysis of vitamin K-1 in fruits and vegetables using accelerated solvent extraction and liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization

作者：Japelt Rie Bak; Jakobsen Jette^*

来源：Food Chemistry, 2016, 192: 402-408.

DOI：10.1016/j.foodchem.2015.06.111

• Vitamin K-1
• Fruits and vegetables
• Accelerated solvent extraction
• LC-MS/MS
• APCI

The objective of this study was to develop a rapid, sensitive, and specific analytical method to study vitamin K-1 in fruits and vegetables. Accelerated solvent extraction and solid phase extraction was used for sample preparation. Quantification was done by liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization in selected reaction monitoring mode with deuterium-labeled vitamin K1 as an internal standard. The precision was estimated as the pooled estimate of three replicates performed on three different days for spinach, peas, apples, banana, and beetroot. The repeatability was 5.2% and the internal reproducibility was 6.2%. Recovery was in the range 90-120%. No significant difference was observed between the results obtained by the present method and by a method using the same principle as the CEN-standard i.e. liquid-liquid extraction and post-column zinc reduction with fluorescence detection. Limit of quantification was estimated to 0.05 mu g/100 g fresh weight.

• 出版日期2016-2-1