Using environmental DNA to detect an endangered crayfish Cambaroides japonicus in streams

作者：Ikeda Kousuke; Doi Hideyuki; Tanaka Kazunori; Kawai Tadashi; Negishi Junjiro N

来源：Conservation Genetics Resources, 2016, 8(3): 231-234.

DOI：10.1007/s12686-016-0541-z

• Species-specific primers
• Real-time PCR
• Lotic system
• Species detection

The freshwater crayfish, Cambaroides japonicus, is endangered in Hokkaido, Japan and inhabits burrows. Here, we applied environmental DNA (eDNA) method for evaluating the species distributions with comparing hand-capture method in the headwater streams. We detected the eDNA of C. japonicus from all sites, where we collected C. japonicus, and confirmed that eDNA can be applied to detect burrowing aquatic freshwater crayfish without disturbing their habitats.

• 出版日期2016-9