Absence of macrophage inflammatory protein-1 alpha does not impact macrophage accumulation in adipose tissue of diet-induced obese mice

作者：Surmi Bonnie K; Webb Corey D; Ristau Alexander C; Hasty Alyssa H^*

来源：American Journal of Physiology - Endocrinology And Metabolism, 2010, 299(3): E437-E445.

DOI：10.1152/ajpendo.00050.2010

• chemokine
• adipose tissue macrophage
• macrophage inflammatory protein-1 beta
• regulated upon activation
• normal T cell expressed and secreted

Surmi BK, Webb CD, Ristau AC, Hasty AH. Absence of macrophage inflammatory protein-1 alpha does not impact macrophage accumulation in adipose tissue of diet-induced obese mice. Am J Physiol Endocrinol Metab 299: E437-E445, 2010. First published June 15, 2010; doi:10.1152/ajpendo.00050.2010.-Macrophages and T-lymphocytes are known to accumulate in the white adipose tissue (WAT) of obese mice and humans, but the factors that cause this infiltration are not yet determined. Chemokines, which attract leukocytes to inflammatory sites, are candidates for this process. Macrophage inflammatory protein-1 alpha (MIP-1 alpha) expression is significantly elevated in WAT of obese mice and humans and positively correlates with fasting plasma insulin, but its potential role in leukocyte recruitment to WAT is unknown. MIP-1 alpha-deficient, heterozygous, and wild-type mice were fed a Western diet (WD) for 16 wk. Plasma lipids, adipose tissue mass, energy expenditure, food intake, liver triglyceride content, and inflammatory cytokine expression were not different among genotypes. Gene expression of macrophage markers F4/80 and CD68, as well as T-lymphocyte marker CD3 epsilon was increased in perigonadal WAT of obese WD-fed mice but was not influenced by MIP-1 alpha expression level. Immunohistochemical analysis of WAT also showed no effect of MIP-1 alpha on macrophage content. Two related chemokines, MIP-1 beta and RANTES, had reduced expression in obese male MIP-1 alpha-deficient mice compared with wild-type controls (P <= 0.05). In mice fed the WD for 6 wk, WAT macrophage content was unchanged; however, CD8+ T-lymphocytes accumulated to a lesser extent in the MIP-1 alpha-null mice. Therefore, expression of MIP-1 alpha, as well as that of MIP-1 beta and RANTES, increases as a consequence of weight gain, but these chemokines may not be required for the recruitment of monocytes to WAT during diet-induced obesity in mice and may impact T-lymphocyte recruitment only at early time points after WD feeding.