A Novel Optical Biosensing System Using Mach-Zehnder-Type Optical Waveguide for Influenza Virus Detection

作者：Sakamoto Hiroaki; Minpou Yuma; Sawai Takayuki; Enami Yasufumi; Suye Shin ichiro^*

来源：Applied Biochemistry and Biotechnology, 2016, 178(4): 687-694.

DOI：10.1007/s12010-015-1902-x

• Molecular immobilization
• Optical biosensing
• Influenza virus detection

In order to minimize the damage from viral epidemics, early detection of the causative agent of a viral epidemic and prevention of its immediate spread are urgent social demands. Therefore, in this study, we evaluated the utility of a Mach-Zehnder-type optical waveguide as a sensing device for influenza virus detection. However, it is impossible to detect a 100-nm-size virus using a sol-gel optical biosensor because sol-gel glass has a pore size of only a few nanometers, which makes it impossible for the virus to diffuse into the silica thin film. In order to construct the influenza-specific Mach-Zehnder optical biosensor for influenza detection, a stable antibody immobilization method with resulting high density on the sol-gel surface is strongly required. In this study, the sol-gel glass surface was modified with amino and carboxyl groups, and an anti-H1N1/HA1 antibody was covalently immobilized using a cross-linking agent. We successfully prepared a carboxyl-modified sol-gel surface, using NHS/EDC as the cross-linker, for antibody immobilization, and confirmed the detection of influenza virus using the antibody-immobilized sol-gel glass. After treatment with a 100 mu g/mL influenza virus solution for 15 min, a peak wavelength shift (similar to 24 nm) was observed in the output light spectrum.

• 出版日期2016-2