Neuroprotective Consequences of Postconditioning on Embolic Model of Cerebral Ischemia in Rat

作者：Rezazadeh Hosseinali; Kahnuee Mohammadamin Hoseini; Roohbakhsh Ali; Shamsizadeh Ali; Rahmani Mohammad Reza; Bidaki Reza; Amin Fatemeh; Kamali Bahareh; Bakhshi Hamid; Allahtavakoli Mohammad^*

来源：Iranian Journal of Basic Medical Sciences, 2013, 16(2): 144-149.

• Cerebral blood flow
• Embolic stroke
• Postconditioning

Objective(s): It has been reported that ischemic postconditioning, conducted by a series of brief occlusion and release of the bilateral common carotid arteries, confers neuro-protection in permanent or transient models of stroke. However, consequences of postconditioning on embolic stroke have not yet been investigated.
Materials and Methods: In the present study, rats were subjected to embolic stroke (n=30) or sham stroke (n=5). Stroke animals were divided into control (n=10) or three different patterns of postconditioning treatments (n=20). In the first pattern of postconditioning (PC10, n=10), the common carotid arteries (CCA) were occluded and reopened 10 and 30 sec, respectively for 5 cycles. Both occluding and releasing times in pattern 2 (PC30, n=5) and 3 (PC60, N=5) of postconditionings, were five cycles of 30 or 60 sec, respectively. Postconditioning was induced at 30 min following the stroke. Subsequently, cerebral blood flow (CBF) was measured from 5 min before to 60 min following to stroke induction. Infarct size, brain edema and neurological deficits and reactive oxygen species (ROS) level was measured two days later.
Results: While PC10 (P<0.001), PC30 and PC60 (P<0.05) significantly decreased infarct volume, only PC10 decreased brain edema and neurological deficits (P<0.05). Correspondingly, PC10 prevented the hyperemia of brain at 35, 40, 50 and 60 min after the embolic stroke (P<0.005). No significant difference in ROS level was observed between PC10 and control group.
Conclusion: Ischemic postconditioning reduces infarct volume and brain edema, decreases hyperemia following to injury and improves neurological functions after the embolic model of stroke.

• 出版日期2013-2