The Olfactory Organ of the Trout Salmo trutta fario: A Novel Localization for a Progestin Receptor

作者：Varricchio Ettore; Coccia Elena; Putti Rosalba; Paolucci Marina^*

来源：Microscopy Research and Technique, 2010, 73(3): 206-214.

DOI：10.1002/jemt.20776

• olfactory organ
• trout
• progesterone

A progestin receptor (PR) has been detected. in the olfactory organ of the trout Salmo trutta fario. The specificity of this receptor was high for 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-DP), but it also bound 17 alpha-hydroxy-progesterone (17 alpha-OHP) and 21-hydroxyprogesterone (21-OHP), even when present at low concentrations (10-fold in relative binding affinity assay). Progesterone (P) competed effectively at much higher concentrations (1,000-fold in relative binding affinity assay). Immunohistochemical studies carried out with three different monoclonal antibodies against human progesterone receptor (hPR), chicken progesterone receptor hinge region (cPR), and chicken progesterone receptor A/B domain (PR22), revealed that immunoreactivity was present in the epithelium of the olfactory organ of females and males of the trout Salmo trutta fario only against hPR. Western blotting showed two hPR immunoreactive bands of about 62 and 66 kDa. Finally, a portion of the cDNA of about 300 nucleotides extending over the DNA binding domain and the ligand binding domain was cloned and sequenced, revealing a high degree of sequence homology of the PR in Salmo trutta fario with the PR in other teleosts. Microsc. Res. Tech. 73:206-214, 2010.

• 出版日期2010-3