A CCD-Based Reader Combined Quantum Dots-Labeled Lateral Flow Strips for Ultrasensitive Quantitative Detection of Anti-HBs Antibody

作者：Zhang, Xueqing; Li, Ding; Wang, Can; Zhi, Xiao; Zhang, Chunlei; Wang, Kan; Cui, Daxiang^*

来源：Journal of Biomedical Nanotechnology, 2012, 8(3): 372-379.

DOI：10.1166/jbn.2012.1401

• CCD
• Lateral Flow Chip
• Quantum Dots
• HBV
• Surface Antigen
• Antibody
• Quantitative Analysis

Herein we reported a CCD-based reader combined quantum dots-labeled lateral flow strips for ultrasensitive quantitative detection of anti-HBs antibody. The CdTe quantum dots were prepared, then were used to label Hepatitis B Virus surface antigen, and then were fabricated into lateral flow strips. The as-prepared lateral flow strips were used to test different concentration of anti-HBV surface antibodies. The CCD-based reader was designed and fabricated, the quantitative analysis software was compiled, and resultant CCD-based reader system was used for quantitative analysis of examined anti-HBs antibodies on the strips. Results showed that the quantum dots-labeled lateral flow strips could detect the anti-HBs antibody with the limitation concentration of 200 pg/mL, the CCD-based reader system could detect anti-HBs antibody with the sensitivity of 2 pg/mL. In conclusion, the prepared CCD-based reader combined quantum dots-labeled lateral flow strips can be used for quantitative detection of anti-HBs antibody in sera with the sensitivity of 2 pg/mL, and has great potential in applications such as ultrasensitive detection of HBV antigens or antibodies, and other tumor biomarkers in near future.

• 出版日期2012-6
• 单位上海交通大学; 中国人民解放军第二六一医院