The phenyl-thiophenyl propenone RK-I-123 reduces the levels of reactive oxygen species and suppresses the activation of NF-kappa B and AP-1 and IL-8 expression in Helicobacter pylori-infected gastric epithelial AGS cells

作者：Jang Sung Hee; Cho Soonok; Lee Eung Seok; Kim Jung Mogg; Kim Hyeyoung^*

来源：Inflammation Research, 2013, 62(7): 689-696.

DOI：10.1007/s00011-013-0621-4

• Helicobacter pylori
• RK-I-123
• IL-8
• NF-kappa B
• Gastric epithelial cells

To investigate whether the phenyl-thiophenyl propenone RK-I-123 suppresses interleukin-8 (IL-8) expression and activation of mitogen-activated protein kinases (MAPKs) and transcription factors (nuclear factor-kappa B [NF-kappa B] and activator protein-1 [AP-1]) by reducing reactive oxygen species (ROS) levels in Helicobacter pylori-infected gastric epithelial cells. %26lt;br%26gt;Helicobacter pylori in Korean isolates, human gastric epithelial AGS cells. %26lt;br%26gt;AGS cells pretreated with or without RK-I-123 were cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1. %26lt;br%26gt;Reactive oxygen species and IL-8 levels were determined by dichlorofluorescein fluorescence and enzyme-linked immunosorbent assay. The IL-8 mRNA expression was analyzed by the real-time reverse transcription-polymerase chain reaction (RT-PCR). The MAPK and I kappa B alpha levels were determined by western blotting. The activation of NF-kappa B and AP-1 was determined by the electrophoretic mobility shift assay. %26lt;br%26gt;Helicobacter pylori induced an increase in ROS and IL-8 expression and activation of MAPKs and transcription factors (NF-kappa B and AP-1) together with the degradation of I kappa B alpha in AGS cells, all of which were inhibited by RK-I-123. %26lt;br%26gt;The RK-I-123 suppressed the H. pylori-induced IL-8 expression and activation of MAPKs, NF-kappa B, and AP-1 by reducing ROS levels in AGS cells. The RK-I-123 may be a potential candidate for the treatment of H. pylori-induced gastric inflammation.

• 出版日期2013-7