In vitro Germination of Early Ripening Sweet Cherry Varieties (Prunus avium L.) at Different Fruit Ripening Stages

作者：Dulic Jovana^*; Ognjanov Vladislav; Ercisli Sezai; Miodragovic Maja; Barac Goran; Ljubojevic Mirjana; Doric Dusica

来源：Erwerbs-Obstbau, 2016, 58(2): 113-118.

DOI：10.1007/s10341-016-0265-y

• Enbryo culture
• Early ripening
• In vitro Prunus avium L
• Sweet cherry
• Stratification

In vitro embryo culture enabled satisfactory germination of immature seeds produced in crosses from early ripening sweet cherry varieties (Prunus avium L.). Three varieties -'Rita', 'Bigarreau Burlat' and 'Carmen'- were crossed with 'Early Star' as male parent. Germination rate was affected by the developmental stage of both fruit and embryo. Fruit ripening stage was a critical factor for culture infection rate that increased with maturity. In-ovule embryo culture on Murashige and Skoog medium without hormones improved the embryo size but did not increase the germination rate due to a further increase in infection rate. Ex-ovule embryo culture on Murashige and Skoog medium supplemented with BA 1 mg L-1, NAA 0.5 mg L-1, 20 g L(-1)sucrose, 10 g L-1 sorbitol and 6 g L(-1)agar during the stratification time increased embryo length. Germination was performed on Brooks and Hough medium at the 22 +/- 1 A degrees C with 16/8 h light/dark photoperiod. The highest germination rate (75 %) was reached in embryos that were 3-4 mm in length, after 30-days stratification at 4 A degrees C. Embryos in fruits at green-yellow stage that were 3-4 mm long were morpho-physiologically developed to produce bipolar seedlings, without combined application of embryo culture and micropropagation.

• 出版日期2016-6