Marinicauda pacifica gen. nov., sp nov., a prosthecate alphaproteobacterium of the family Hyphomonadaceae isolated from deep seawater

作者：Zhang, Xi-Ying^*; Li, Guo-Wei; Wang, Chun-Sheng; Zhang, Yan-Jiao; Xu, Xue-Wei; Li, Hai; Liu, Ang; Liu, Chang; Xie, Bin-Bin; Qin, Qi-Long; Xu, Zhong; Chen, Xiu-Lan; Zhou, Bai-Cheng; Zhang, Yu-Zhong

来源：International Journal of Systematic and Evolutionary Microbiology, 2013, 63: 2248-2253.

DOI：10.1099/ijs.0.046656-0

• DEOXYRIBONUCLEIC-ACID
• EMENDED DESCRIPTION
• BACTERIUM
• DATABASE

A marine prosthecate bacterium, designated strain P-1 km-3(T), was isolated from deep seawater from the Pacific. Cells of strain P-1 km-3(T) were Gram-stain-negative, aerobic, catalase- and oxidase-positive, dimorphic rods with a single polar prostheca or flagellum. The strain hydrolysed gelatin and grew at 6-40 degrees C (optimum, 30 degrees C) and with 0.5-12% (w/v) NaCl (optimum, 2%). Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain P-1 km-3T belonged to the family Hyphomonadaceae in the class Alphaproteobacteria and represented a separate lineage, located between the genera Oceanicaulis and Woodsholea. Sequence similarities of strain P-1 km-3T with type strains of species of the genera Oceanicaulis and Woodsholea were 93.2-93.9 %. The predominant cellular fatty acids in strain P-1 km-3(T) were C-18:1 omega 7c, C-18:0, 11-methyl C-18:1 omega 7c, C-17:0 and C-19:0 cyclo omega 8c. The major respiratory quinone of strain P-1 km-3(T) was Q-10. The polar lipids of strain P-1 km-3(T) comprised glucuronopyranosyldiglyceride (GUDG), monoglycosyldiglyceride (MGDG), sulfo-quinovosyl diacylglycerol (SQDG), phosphatidylglycerol (PG), an unidentified phospholipid (PL) and an unidentified lipid (L). The genomic DNA G C content of strain P-1 km-3T was 66.0 mol%. On the basis of the polyphasic data presented in this study, strain P-1 km-3(T) is proposed to represent a novel species in a new genus, Marinicauda pacifica gen. nov., sp. nov., within the family Hyphomonadaceae. The type strain of the type species is P-1 km-3(T) (=KACC 16526(T)=CGMCC 1.11031(T)).

• 出版日期2013-6
• 单位山东大学; 国家海洋局第二海洋研究所; 微生物技术国家重点实验室