Development of mediated BOD biosensor system of flow injection mode for shochu distillery wastewater

作者：Oota Shinichi; Hatae Yuta; Amada Kei; Koya Hidekazu; Kawakami Mitsuyasu^*

来源：Biosensors and Bioelectronics, 2010, 26(1): 262-266.

DOI：10.1016/j.bios.2010.06.040

• Biochemical oxygen demand
• Flow injection
• Mediator
• Shochu distillery wastewater

Although microbial biochemical oxygen demand (BUD) sensors utilizing redox mediators have attracted much attention as a rapid BUD measurement method, little attempts have been made to apply the mediated BOD biosensors to the flow injection analysis system In this work, a mediated BUD sensor system of flow injection mode, constructed by combining an immobilized microbial reactor with an electrochemical flow cell of three electrodes configuration, has been developed to estimate BUD of shochu distillery wastewater (SDW). It was demonstrated consequently that the mediated sensing was realized by employing phosphate buffer containing potassium hexacyanoferrate as the carrier. The output current was found to yield a peak with a sample injection, and to result from reoxidation of reduced mediator at the electrode By employing the peak area as the sensor response, the effects of flow rate and pH of the carrier on the sensitivity were investigated.

• 出版日期2010-9-15