A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector

作者：Huang, Hongliang; Yu, Hai; Li, Da; Liu, Yang; Shen, Fenping; Zhou, Jun; Wang, Qingqing^*; Tang, Guping

来源：International Journal of Molecular Sciences, 2008, 9(11): 2278-2289.

DOI：10.3390/ijms9112278

• Polyethylenimine
• 2-hydroxypropyl-alpha-cyclodextrin
• non-viral gene delivery vector
• co-polymer

A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.

• 出版日期2008-11
• 单位浙江大学; 广东药科大学